What is UV spectroscopy?

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Ultraviolet (UV) spectroscopy is a method of determining which wavelengths (colours) of visible light a sample absorbs or emits. There are three main types of UV spectroscopy: transmittance, absorbance, and emission.

Transmittance and absorbance spectroscopy are opposites of one another. In transmittance spectroscopy, different wavelengths of monochromatic light are shot at a sample, and the wavelengths that do not interact with the sample are measured by a detector on the other side of the sample. In absorbance spectroscopy, the light that is absorbed (that is to say, the light that does interact with the sample) is measured using the same setup. If one were to take a graph of transmittance and invert it, it would be an absorbance graph.

Emission is done in a slightly different way. Often, when molecules absorb light, they are put into a higher energy level (in one way or another, rotationally, vibrationally, electronically, etc.) which is less stable than their ground state. When the molecules returns to its ground state, it releases light of equal wavelength to that it absorbed. The prime difference between the light that is emitted and the light that was absorbed is direction. Emitted light can be released in any direction. Thus, in order to avoid measuring light from the source, rather than only light that has been emitted, detectors in emission spectroscopy are often put at a 90 degree angle from the source light beam.
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What is woodwardfieser rule in uv spectroscopy?

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Principle of UV Visible spectroscopy?

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What are Application of UV spectroscopy in biology?

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How do you get calibration curve for uv-visible spectroscopy?

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What is the difference between uv spectroscopy and uv visible spectroscopy?

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How is UV spectroscopy generally used?

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