What is decoy oligonucleotides?
Transcription Factor oligodeoxynucleotide decoys (ODN decoys). Use our GeneDetect® transcription factor decoys to inhibit specific transcription factors in cell culture. Complete list of Transcription Factor decoy Products. Introduction
Transcription factor ODN decoy approach
Advantages and disadvantages of the ODN decoy approach for studying cellular gene expression
ODN Decoys available from GeneDetect.com
How are these decoys used?
Cells can respond to stimuli (normal or pathological) by changing the levels of expression of specific genes. The cellular proteins that regulate changes in gene expression are called transcription factors. Transcription factors are generally nuclear and can either be constitutively expressed within the cell (present under basal conditions, for example CREB) or themselves inducible (for example AP-1). These transcription factor proteins bind specific sequences found in the promoter regions of genes (target genes) whose expression they then regulate (switch on or off). These binding sequences are generally 6-10 base pairs in length and are occasionally found in multiple copies within the promoter regions of target genes. Although the transcription factor protein-DNA interaction is sequence-specific, the binding site for one given transcription factor may vary by several base pairs within different target genes. Therefore when we describe the specific DNA binding sequence for a transcription factor we refer to the non-variable part of the binding sequence, that is, the transcription factor consensus sequence. For example, the AP-1 transcription factor made up of Fos and Jun proteins binds to the TGACTCA consensus sequence. In comparison the consensus sequence for the Smad transcription factor family which mediate TGF-b, activin and BMP induced changes in gene expression is CAGACA. Fig. 1. Transcription factor ODN decoy approach. The basic theory behind the transcription factor ODN decoy approach involves flooding the cell with competing synthetic, transcription factor-specific consensus sequences. These synthetic decoys "compete" for binding of the transcription factor with consensus sequences in target genes. If delivered into the cell in sufficient concentrations these "decoys" thus have the potential to attenuate the binding of the transcription factor to promoter regions of target genes and thus attenuate the function of the transcription factor to regulate the expression of its target gene(s). Transfected at high concentrations these decoys have been reported in the literature to completely block transcription factor function. Clearly they represent powerful research tools for studying gene regulation both in vitro and also more recently in vivo (for Reviews see Moshita et al., 1998, Mann and Dzau, 2000). Fig. 2. Advantages and disadvantages of the ODN decoy approach for studying cellular gene expression. Advantages.
- ODN decoys offer a means of specifically inhibiting transcription factor function in living cells.
- Inexpensive compared to other more classical methods of investigating gene expression such as chloramphenicol acetyltransferase and luciferase constructs in promoter-reporter gene transfection experiments.
- Allows for investigation of both endogenous and pathological gene regulation
- Proven to be highly effective and selective within in vitro experiments.
- Easy to use.
- An emerging technology that has not yet been fully characterized
- Issues of decoy synthesis. High levels of purity and stability required.
- Transfection issues. Which method is best. How to optimize transfection.
- Issue of controls. What controls are needed.
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Non radio active labeling for oligonuleotides include:. Biotin-labelled oligonucleotides - this biotin can be used to link the oligonucleotide to streptavidin-protein conjug…ates, streptavidin affinity columns, or labelled streptavidin. Attachment of a biotin molecule to the 3' terminus can also be used to prevent 3' exonuclease digestion and 3' extension during amplification reactions.. Digoxigenin (DIG) is mainly used as a non-isotopic label for DNA and oligonucleotides in a wide range of applications: diagnostics, sequencing, blot applications, and in situ hybridization. The digoxigenin label can be detected with enzyme-linked immunoassays for anti-digoxigenin protein conjugates, and visualized through a chemiluminescent reaction.. DNP means 2,4-Dinitrophenyl. DNP can be introduced anywhere in the sequence and on either terminus.DNP is an alternative to digoxigenin in cases where two different genes must be detected together. Oligonucleotides labelled with DNP can therefore be used in analytical tests based on anti-DNP antibodies.. For other non radioactive labeling, check this site: www.biosyn.com, they offer various oligonucleotide modifications and labelings.
Yes, presidents do have decoys incase the White House in under attack, or incase some one is trying to assasinate the president.
ang antonym ng decoy ay kana!
is a grouping of soiled tablewares in soiled dish table according to size and shape
To draw attention away from something in particular, to distract.
I believe there is no CAS No for oligonucleotide. However, here's the CAS No for DNA and RNA. Hope it will be helpful. Deoxyribonucleic Acid CAS No. 9007-49-2 Ribonucleic Aci…d CAS No. 66082-38-0
A Decoy Detonator is a black horn-like object. When dropped, it runs a reasonable distance away and makes a loud bang noise. It then releases clouds of black smoke. It is used… as a diversion and was invented by Fred and George Weasley. They are sold at Weasleys' Wizard Wheezes.
No. A decoy is created by a Pokemon to save it from harm, it is not a Pokemon itself.
fake made up person or thing oftrn used as a direction decoy
Allele specific PCR is used in the process of DNA fingerprinting where specific alleles Can be amplifies and the degree of similarity or difference between individuals can be …determined.
For example, when the outside hitter comes behind the middle blocker and hits, the middle blocker will jump up while the outside hitter behind the middle hits the ball. The Mi…ddle blocker acts as a decoy to trick the blockers on the opposing team.
by the time you find out it's too late because it is in fact lethal
A decoy is 'bait', you leave a piece purposefully undefended, toget the opponent to perform a specific action. That action openshis own defences up for an attack.
Oligonucleotide are single-strand and short DNA. Oligonucleotide has a use in the areas of forensics, genetic testing and research in the field of science.
Decoys was released on 02/27/2004.